AimMy aim is to see how the permeability of beetroot membranes are affected by a change in temperature. Equipment
Method
SafetyThe water baths above 50 degrees are hot and therefore can burn so you must be careful when handling them. When using the scalpel to cut the beetroot, you must be careful not to cut your hand. ResultsQuestionList the variables that were controlled during the experiment and state how they were controlled.
Shape and size of beetroot samples can be controlled by using a ruler to perfectly get 1cm samples. Also it is important to cut straight down and not at an angle so the surface area is not changed. The cork bore also produces perfect cylinders. Time left in water bath - were left in bath for 15 minutes, this was measured with a stopwatch Suggest why the tubes were placed in the water baths for 5 minutes before the cylinders were added This was to allow the water in the test tubes to reach the same temperature as in the water bath Why were the beetroot cylinders washed with distilled water and dried before the experiment This was to remove pigment that has come out from the beetroot in the process of extracting cylinders and cutting into 1cm lengths. Use the trend line of your graph to describe the effect of temperature on the percentage transmission between 30°C and 80°C Explain your results in detail in terms of what is happening to the beetroot membrane My class and I found a negative correlation between temperature and transmission, meaning as the temperature rose, less light was able to pass through the solution. This is because at higher temperatures, the phospholipid membrane becomes more fluid and 'leakier', meaning more pigment is able to escape. It also means that the proteins in the membrane denature and allow more pigment to pass through.
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